MALDI Tissue Imaging of Drugs with the ultraflex MALDI TOF/TOF

MALDI-TOF MS has previously been used for imaging of large peptides and proteins embedded in tissue sections. This imaging technique is not limited to larger molecules, but can also be used to measure the presence of small molecules directly from tissue. However, for small molecule imaging the method of choice is a selected reaction monitoring approach in MS/MS mode.The benefits of parent ion isolation and subsequent detection of fragment ions by MALDI-TOF/TOF can alleviate matrix interference, and provide enhanced sensitivity due to the specificity of parent ion isolation. Additionally, since the smartbeamTM laser diameter is five to ten times smaller than traditional lasers (1), the ultraflex IITM MALDI-TOF/TOF provides superior high resolution images of small molecules. It should be noted that the experimental conditions described for this Olanzapine (OLZ) study may not equally apply to other drug molecules. It has Application Note # MT-87 MALDI Tissue Imaging of Drugs with the ultraflex MALDI TOF/TOF